Virus:Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD68 antibody Abcam cat#ab845 anti-TREM2 antibody Novus Biologicals Clone#37920 Bacterial and virus strains lentiviral vector lenti_dCas9-KRAB-MeCP2 Andrea Califano # 122205; http://n2t.net/addgene:122205; RRID: Addgene_122205 pSPgRNA vector Charles Gersbach Addgene plasmid # 47108; http://n2t.net/ addgene:47108; RRID: Addgene_47108 Biological samples PERIHEART right atrial appendage samples PERIHEART N/A PERIHEART pericardial fluid samples PERIHEART N/A CAREBANK right atrial appendage samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 CAREBANK serum samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 Chemicals, peptides, and recombinant proteins IL-1b 10 ng/ml GIBCO Cat#PHC0814 Hematoxylin Vector Laboratories Cat#H-3401-500 Eosin Sigma-Aldrich Cat#HT110116 EcoMount mounting medium BioCare Medical Cat#EM897L EGM; basal medium Lonza Cat#CC-4133 Fetal bovine serum (FBS) GIBCO Cat#A3840001 ACK lysing buffer GIBCO, Thermo Fisher Scientific, Inc Cat#A1049201 RNase inhibitors TaKaRa Cat#2313A EDTA-free protease inhibitor cocktail Roche Cat#11873580001 BODIPYTM 493/503 (4,4-Difluoro-1,3,5,7,8Pentamethyl-4-Bora-3a,4a-Diaza-sIndacene) ThermoFisher Cat#D3922 Vascular Cell Basal Medium ATCC Cat#PCS-100-030 Vascular Endothelial Cell Growth Kit-VEGF ATCC Cat#PCS-100-041 Resuspension buffer R Thermo Fisher Sci. .. Cat# MPK1096 Quick-DNA/RNA Miniprep Plus Kit Zymo Cat#D7003 Blasticidin S HCL Corning, Fisher Scientific Cat#15383671 Monarch Total RNA Miniprep Kit NEB Cat#T2010 Critical commercial assays Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns 10x Genomics Cat#PN-1000075 Dual Index kit TT set A 10x Genomics Cat#PN-1000215 NovaSeq 6000 S2 System Illumina N/A RNeasy Mini Kit QIAGEN Cat#74104 Molecular CartographyTM spatial transcriptomics Resolve Bioscience N/A Visium Spatial Gene Expression Slide & Reagent Kit, 16 rxns 10x Genomics Cat#PN-1000184 Visium Spatial Tissue Optimization Slide & Reagent Kit, 4 slides 10x Genomics Cat#PN-1000193 NextSeq 500 system (150 cycles) Illumina N/A (Continued on next page) Cell Reports Medicine 5, 101556, May 21, 2024 e1
Plasmid Preparation:Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD68 antibody Abcam cat#ab845 anti-TREM2 antibody Novus Biologicals Clone#37920 Bacterial and virus strains lentiviral vector lenti_dCas9-KRAB-MeCP2 Andrea Califano # 122205; http://n2t.net/addgene:122205; RRID: Addgene_122205 pSPgRNA vector Charles Gersbach Addgene plasmid # 47108; http://n2t.net/ addgene:47108; RRID: Addgene_47108 Biological samples PERIHEART right atrial appendage samples PERIHEART N/A PERIHEART pericardial fluid samples PERIHEART N/A CAREBANK right atrial appendage samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 CAREBANK serum samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 Chemicals, peptides, and recombinant proteins IL-1b 10 ng/ml GIBCO Cat#PHC0814 Hematoxylin Vector Laboratories Cat#H-3401-500 Eosin Sigma-Aldrich Cat#HT110116 EcoMount mounting medium BioCare Medical Cat#EM897L EGM; basal medium Lonza Cat#CC-4133 Fetal bovine serum (FBS) GIBCO Cat#A3840001 ACK lysing buffer GIBCO, Thermo Fisher Scientific, Inc Cat#A1049201 RNase inhibitors TaKaRa Cat#2313A EDTA-free protease inhibitor cocktail Roche Cat#11873580001 BODIPYTM 493/503 (4,4-Difluoro-1,3,5,7,8Pentamethyl-4-Bora-3a,4a-Diaza-sIndacene) ThermoFisher Cat#D3922 Vascular Cell Basal Medium ATCC Cat#PCS-100-030 Vascular Endothelial Cell Growth Kit-VEGF ATCC Cat#PCS-100-041 Resuspension buffer R Thermo Fisher Sci. .. Cat# MPK1096 Quick-DNA/RNA Miniprep Plus Kit Zymo Cat#D7003 Blasticidin S HCL Corning, Fisher Scientific Cat#15383671 Monarch Total RNA Miniprep Kit NEB Cat#T2010 Critical commercial assays Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns 10x Genomics Cat#PN-1000075 Dual Index kit TT set A 10x Genomics Cat#PN-1000215 NovaSeq 6000 S2 System Illumina N/A RNeasy Mini Kit QIAGEN Cat#74104 Molecular CartographyTM spatial transcriptomics Resolve Bioscience N/A Visium Spatial Gene Expression Slide & Reagent Kit, 16 rxns 10x Genomics Cat#PN-1000184 Visium Spatial Tissue Optimization Slide & Reagent Kit, 4 slides 10x Genomics Cat#PN-1000193 NextSeq 500 system (150 cycles) Illumina N/A (Continued on next page) Cell Reports Medicine 5, 101556, May 21, 2024 e1
Recombinant:Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD68 antibody Abcam cat#ab845 anti-TREM2 antibody Novus Biologicals Clone#37920 Bacterial and virus strains lentiviral vector lenti_dCas9-KRAB-MeCP2 Andrea Califano # 122205; http://n2t.net/addgene:122205; RRID: Addgene_122205 pSPgRNA vector Charles Gersbach Addgene plasmid # 47108; http://n2t.net/ addgene:47108; RRID: Addgene_47108 Biological samples PERIHEART right atrial appendage samples PERIHEART N/A PERIHEART pericardial fluid samples PERIHEART N/A CAREBANK right atrial appendage samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 CAREBANK serum samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 Chemicals, peptides, and recombinant proteins IL-1b 10 ng/ml GIBCO Cat#PHC0814 Hematoxylin Vector Laboratories Cat#H-3401-500 Eosin Sigma-Aldrich Cat#HT110116 EcoMount mounting medium BioCare Medical Cat#EM897L EGM; basal medium Lonza Cat#CC-4133 Fetal bovine serum (FBS) GIBCO Cat#A3840001 ACK lysing buffer GIBCO, Thermo Fisher Scientific, Inc Cat#A1049201 RNase inhibitors TaKaRa Cat#2313A EDTA-free protease inhibitor cocktail Roche Cat#11873580001 BODIPYTM 493/503 (4,4-Difluoro-1,3,5,7,8Pentamethyl-4-Bora-3a,4a-Diaza-sIndacene) ThermoFisher Cat#D3922 Vascular Cell Basal Medium ATCC Cat#PCS-100-030 Vascular Endothelial Cell Growth Kit-VEGF ATCC Cat#PCS-100-041 Resuspension buffer R Thermo Fisher Sci. .. Cat# MPK1096 Quick-DNA/RNA Miniprep Plus Kit Zymo Cat#D7003 Blasticidin S HCL Corning, Fisher Scientific Cat#15383671 Monarch Total RNA Miniprep Kit NEB Cat#T2010 Critical commercial assays Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns 10x Genomics Cat#PN-1000075 Dual Index kit TT set A 10x Genomics Cat#PN-1000215 NovaSeq 6000 S2 System Illumina N/A RNeasy Mini Kit QIAGEN Cat#74104 Molecular CartographyTM spatial transcriptomics Resolve Bioscience N/A Visium Spatial Gene Expression Slide & Reagent Kit, 16 rxns 10x Genomics Cat#PN-1000184 Visium Spatial Tissue Optimization Slide & Reagent Kit, 4 slides 10x Genomics Cat#PN-1000193 NextSeq 500 system (150 cycles) Illumina N/A (Continued on next page) Cell Reports Medicine 5, 101556, May 21, 2024 e1
Protease Inhibitor:Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD68 antibody Abcam cat#ab845 anti-TREM2 antibody Novus Biologicals Clone#37920 Bacterial and virus strains lentiviral vector lenti_dCas9-KRAB-MeCP2 Andrea Califano # 122205; http://n2t.net/addgene:122205; RRID: Addgene_122205 pSPgRNA vector Charles Gersbach Addgene plasmid # 47108; http://n2t.net/ addgene:47108; RRID: Addgene_47108 Biological samples PERIHEART right atrial appendage samples PERIHEART N/A PERIHEART pericardial fluid samples PERIHEART N/A CAREBANK right atrial appendage samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 CAREBANK serum samples CAREBANK ClinicalTrials.gov Identifier: NCT03444259 Chemicals, peptides, and recombinant proteins IL-1b 10 ng/ml GIBCO Cat#PHC0814 Hematoxylin Vector Laboratories Cat#H-3401-500 Eosin Sigma-Aldrich Cat#HT110116 EcoMount mounting medium BioCare Medical Cat#EM897L EGM; basal medium Lonza Cat#CC-4133 Fetal bovine serum (FBS) GIBCO Cat#A3840001 ACK lysing buffer GIBCO, Thermo Fisher Scientific, Inc Cat#A1049201 RNase inhibitors TaKaRa Cat#2313A EDTA-free protease inhibitor cocktail Roche Cat#11873580001 BODIPYTM 493/503 (4,4-Difluoro-1,3,5,7,8Pentamethyl-4-Bora-3a,4a-Diaza-sIndacene) ThermoFisher Cat#D3922 Vascular Cell Basal Medium ATCC Cat#PCS-100-030 Vascular Endothelial Cell Growth Kit-VEGF ATCC Cat#PCS-100-041 Resuspension buffer R Thermo Fisher Sci. .. Cat# MPK1096 Quick-DNA/RNA Miniprep Plus Kit Zymo Cat#D7003 Blasticidin S HCL Corning, Fisher Scientific Cat#15383671 Monarch Total RNA Miniprep Kit NEB Cat#T2010 Critical commercial assays Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns 10x Genomics Cat#PN-1000075 Dual Index kit TT set A 10x Genomics Cat#PN-1000215 NovaSeq 6000 S2 System Illumina N/A RNeasy Mini Kit QIAGEN Cat#74104 Molecular CartographyTM spatial transcriptomics Resolve Bioscience N/A Visium Spatial Gene Expression Slide & Reagent Kit, 16 rxns 10x Genomics Cat#PN-1000184 Visium Spatial Tissue Optimization Slide & Reagent Kit, 4 slides 10x Genomics Cat#PN-1000193 NextSeq 500 system (150 cycles) Illumina N/A (Continued on next page) Cell Reports Medicine 5, 101556, May 21, 2024 e1
|
Addgene inc is a verified supplier 